PCNA Antibody

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Immunohistochemistry: PCNA Antibody [NB120-15498] - man tonsil stained with Anti-PCNA antibody

Product Details

Summary
Product Discontinued
View other related PCNA Primary Antibodies

Order Details


    • Catalog Number
      NB120-15498
    • Availability
      Product Discontinued

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PCNA Antibody Summary

Immunogen
Recombinant human PCNA protein
Localization
Nuclear
Marker
Proliferation Marker
Specificity
PCNA
Clonality
Polyclonal
Host
Rabbit
Gene
PCNA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin Neat
Application Notes
IHC-P: Working dilution, ready to use for 10 minutes at RT. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
PCNA Lysate (NBL1-14184)

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and BSA
Preservative
Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PCNA Antibody

  • cyclin
  • DNA polymerase delta auxiliary protein
  • MGC8367
  • proliferating cell nuclear antigen

Background

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCNA Antibody (NB120-15498) (0)

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Product General Protocols

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FAQs for PCNA Antibody (NB120-15498) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PCNA Products

Bioinformatics Tool for PCNA Antibody (NB120-15498)

Discover related pathways, diseases and genes to PCNA Antibody (NB120-15498). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PCNA Antibody (NB120-15498)

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Pathways for PCNA Antibody (NB120-15498)

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PTMs for PCNA Antibody (NB120-15498)

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Research Areas for PCNA Antibody (NB120-15498)

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Blogs on PCNA.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

PCNA (Proliferating cell nuclear antigen, polymerase delta auxiliary protein)
PCNA is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It coordinates the recruitment and association of needed components during both of these processes, both of which are essential for cell cycl...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

PCNA is a Universal Marker of Proliferating Cells
Proliferating cell nuclear antigen (PCNA) is an evolutionarily well-conserved protein found in all eukaryotic species as well as in Archaea. PCNA was first shown to be involved in DNA replication. However PCNA functions are associated with other vital...  Read full blog post.

Using PCNA as an Antibody Marker
PCNA antibodies are useful biomarkers in DNA repair studies. PCNA is one of several proteins essential for the completion of nucleotide excision repair, a multi-stage process involving 20 - 30 proteins, and an important factor in repairing damage and...  Read full blog post.

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Bioinformatics

Gene Symbol PCNA
Entrez