PCAF Antibody

Images

 
Western Blot: PCAF Antibody [NB100-92049] - Analysis of PCAF (F820) pAb at 1:500 dilutionLane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Immunohistochemistry-Paraffin: PCAF Antibody [NB100-92049] - Analyzes of PCAF (F820) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: PCAF Antibody [NB100-92049] - Analysis of PCAF (F820) antibody in extracts from COLO205 cells
Immunohistochemistry-Paraffin: PCAF Antibody [NB100-92049] - Analyzes of PCAF (F820) antibody in paraffin-embedded human breast carcinoma tissue

Product Details

Summary
Product Discontinued
View other related PCAF Primary Antibodies

Order Details


    • Catalog Number
      NB100-92049
    • Availability
      Product Discontinued

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PCAF Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 786-841 of Human PCAF.
Clonality
Polyclonal
Host
Rabbit
Gene
KAT2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PCAF (F820) pAb in extracts from COLO205 cells.
Immunohistochemistry (IHC) analyzes of PCAF (F820) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-92049 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PCAF Antibody

  • CREBBP-associated factor
  • EC 2.3.1.48
  • GCN5
  • GCN5L
  • Histone acetylase PCAF
  • histone acetyltransferase KAT2B
  • Histone acetyltransferase PCAF
  • K(lysine) acetyltransferase 2B
  • KAT2B
  • Lysine acetyltransferase 2B
  • P
  • P/CAFGCN5
  • P300/CBP-associated factorCAF
  • PCAF

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAFII p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCAF Antibody (NB100-92049) (0)

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Review for PCAF Antibody (NB100-92049) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-92049:
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Images Ratings Applications Species Date Details
Western Blot PCAF NB100-92049
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reviewed by:
Kim Lab
WB Human 11/16/2016
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Summary

ApplicationWestern Blot
Sample Tested786O cell lysates,RCC4 Whole cell lysate, brain and spinal cord
SpeciesHuman

Comments

CommentsBlocked with 5% Nonfat Dry Milk in 1x TBS-T, 1 hour at room temperature
Dilution - 1:1000 Ab in 5% BSA in TBS-T solution buffer.
Overnight incubation.

Secondary: Goat Anti-Rabbit IgG, HRP-linked Antibody

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Recent Reviews

5
5
1
4
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2
0
1
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Kim Lab
11/16/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol KAT2B
Entrez