PBX1 Antibody (1C11)

Images

 
Western Blot: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PBX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: PBX1 Antibody (1C11) [NBP2-00872] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PBX1.
Immunohistochemistry-Paraffin: PBX1 Antibody (1C11) [NBP2-00872] - Staining of paraffin-embedded Human liver tissue using anti-PBX1 mouse monoclonal antibody.
Flow Cytometry: PBX1 Antibody (1C11) [NBP2-00872] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PBX1 antibody, and then analyzed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
1C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PBX1 Antibody (1C11) Summary

Immunogen
Full length human recombinant protein of human PBX1(NP_002576) produced in HEK293 cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PBX1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PBX1 Antibody (1C11)

  • DKFZp686B09108
  • Homeobox protein PBX1
  • Homeobox protein PRL
  • MGC126627
  • Pre-B cell leukemia transcription factor-1
  • pre-B-cell leukemia homeobox 1
  • pre-B-cell leukemia transcription factor 1
  • PRL

Background

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PBX1
Entrez