Paxillin [p Ser126] Antibody

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Western Blot: Paxillin [p Ser126] Antibody [NSB1022] - RAW 264.7 cell lysates untreated (1) or treated with LPS (2-6) were resolved on 10% polyacrylamide gel and transferred to PVDF. Membrane blocked with 5% milk-TBST ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Paxillin [p Ser126] Antibody Summary

Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains serine 126. The sequence is conserved in mouse and chicken.
Modification
p Ser126
Marker
Focal Adhesion Marker
Specificity
Paxillin [Ser126]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PXN
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application.
Positive Control
Paxillin Lysate (NBL1-15014)

Reactivity Notes

Mouse and human (100% homologous) paxillin. Chicken (100% homologous) has not been tested, but is expected to react.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Paxillin [p Ser126] Antibody

  • FLJ16691
  • Paxillin
  • PXN

Background

Paxillin is a 68 kDa cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. These cellular events in turn play a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, serine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Serine 126 of paxillin is phosphorylated as a result of Raf stimulation, through the Raf --> MEK --> ERK pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PXN
Entrez