Pax7 Antibody

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Immunohistochemistry-Paraffin: Pax7 Antibody [NBP1-52374] - Analysis of anti-PAX7 antibody with human tonsil at concentration 3 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bt, Ca, Eq, Ha, Mk, Pm, Rb, BvSpecies Glossary
Applications ELISA, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pax7 Antibody Summary

Immunogen
Synthetic peptide CSQRADSIKPGDS from an internal region of human PAX7 (NP_002575.1; NP_039236.1; NP_001128726.1).
Specificity
Human PAX7. This antibody is expected to recognise all three reported isoforms (NP_002575.1; NP_039236.1; NP_001128726.1).
Predicted Species
Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry-Paraffin 3-6 ug/ml
Application Notes
ICC/IF usage is reported in scientific literature (PMID: 26178374). IHC: NBP1-52374 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
Publications
Read Publication using
NBP1-52374 in the following applications:

Reactivity Notes

Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Tamarin. Predicted cross-reactivity based on sequence identity: Elephant (100%), Panda (100%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
TBS (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pax7 Antibody

  • FLJ37460
  • HUP1
  • paired box 7
  • paired box gene 7
  • paired box homeotic gene 7
  • paired box protein Pax-7
  • paired domain gene 7
  • PAX7 transcriptional factor
  • Pax7
  • PAX7B
  • RMS2

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax7 Antibody (NBP1-52374)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
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Bioinformatics

Gene Symbol PAX7
Entrez
Uniprot