Western Blot: Pax3 Antibody [NBP1-32944] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: PAX3 Antibody [NBP1-32944] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PAX3 Antibody [NBP1-32944] - Paraffin-embedded SG xenograft, using antibody at 1:100 dilution.
53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-32944 in the following applications:
Mouse reactivity reported in scientific literature (PMID: 22641621).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Pax3 Antibody
paired box 3
paired box gene 3 (Waardenburg syndrome 1)
paired box homeotic gene 3
paired box protein Pax-3
paired domain gene 3
paired domain gene HuP2
Waardenburg syndrome 1
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post.
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