Pax3 Antibody

Images

 
Western Blot: Pax3 Antibody [NBP1-32944] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: PAX3 Antibody [NBP1-32944] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PAX3 Antibody [NBP1-32944] - Paraffin-embedded SG xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Product Discontinued
View other related Pax3 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-32944
    • Availability
      Product Discontinued

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Pax3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PAX3. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32944 in the following applications:

Publications
Read Publication using
NBP1-32944 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22641621).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
2.86 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pax3 Antibody

  • CDHS
  • HUP2
  • HUP2MGC120384
  • MGC120381
  • MGC120382
  • MGC120383
  • MGC134778
  • paired box 3
  • paired box gene 3 (Waardenburg syndrome 1)
  • paired box homeotic gene 3
  • paired box protein Pax-3
  • paired domain gene 3
  • paired domain gene HuP2
  • Pax3
  • Waardenburg syndrome 1
  • WS1
  • WS3

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax3 Antibody (NBP1-32944)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Mouse
(1)
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Review for Pax3 Antibody (NBP1-32944) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32944:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 03/03/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse/human melanocytes
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pax3 Antibody (NBP1-32944) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Pax3 Products

Bioinformatics Tool for Pax3 Antibody (NBP1-32944)

Discover related pathways, diseases and genes to Pax3 Antibody (NBP1-32944). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Pax3 Antibody (NBP1-32944)

Discover more about diseases related to Pax3 Antibody (NBP1-32944).
 

Pathways for Pax3 Antibody (NBP1-32944)

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PTMs for Pax3 Antibody (NBP1-32944)

Learn more about PTMs related to Pax3 Antibody (NBP1-32944).
 

Research Areas for Pax3 Antibody (NBP1-32944)

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Blogs on Pax3.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Recent Reviews

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1
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Anonymous
03/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PAX3
Entrez
Uniprot