PARP [p Asp214] Antibody


Immunoprecipitation: PARP [p Asp214] Antibody [NBP1-50751] - Immunoprecipitation of HEK293 cells treated with etoposide (5h). Image from verified customer review.

Product Details

Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IP, ICC
0.2 mg/ml

Order Details

PARP [p Asp214] Antibody Summary

Synthetic peptide corresponding to the C-terminus surrounding Asp214 of rat PARP
p Asp214
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS, 1.0% BSA, and 50% Glycerol
0.02% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


  • Western Blot 1:100-1:2000
  • Immunohistochemistry 10-20 ug/ml
  • Immunoprecipitation
  • Immunocytochemistry 10 - 20 ug/ml
Application Notes
The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. IP data from customer review.
Reviewed Applications
Read 1 Review rated 4
NBP1-50751 in the following applications:

Reactivity Notes

Human reactivity from customer review.

Alternate Names for PARP [p Asp214] Antibody

  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
  • ADPRT 1
  • ADPRTADP-ribosyltransferase NAD(+)
  • EC 2.4.2
  • EC
  • NAD(+) ADP-ribosyltransferase 1
  • PARP apoptosis
  • PARP1
  • PARP-1
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly [ADP-ribose] polymerase 1
  • poly(ADP-ribose) polymerase
  • poly(ADP-ribose) synthetase
  • poly(ADP-ribosyl)transferase
  • Poly[ADP-ribose] synthase 1
  • PPOL


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Ca, Ge
Applications: IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Mu
Applications: WB, Simple Western, IP
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Simple Western, IHC-Fr, IHC-P, IP
Species: Hu, Rt
Applications: WB, IHC, IP, ICC

Publications for PARP Antibody (NBP1-50751) (0)

There are no publications for PARP Antibody (NBP1-50751).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PARP Antibody (NBP1-50751) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-50751:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation PARP NBP1-50751
reviewed by:
IP Human 08/26/2014


Sample TestedHEK293 cells treated with etoposide (5h)
CommentsThere is some background but the signal is pretty consistent.


Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary Descriptionanti rabbit HRP-conjugated antibody
Secondary Manufacturer Cat#(Life Technologies # 65-6120)
Secondary Concentration1:20,000


Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 2 min exposure using X-ray film
Immunoprecipitation DetailsProtein G PLUS-Agarose (Santa Cruz), 20ul, 4h at 4 degree, washed thrice with PBS containing 0.1% Triton X-100
Elution DescriptionEluted by boiling in 1X SDS dye for 10 minutes


CommentsThere is some background but the signal is pretty consistent.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PARP Antibody (NBP1-50751). (Showing 1 - 2 of 2 FAQ).

  1. I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
    • For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
  2. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies


Isotype Controls

Additional PARP [p Asp214] Antibody Products

Related Products by Gene

Bioinformatics Tool for PARP Antibody (NBP1-50751)

Discover related pathways, diseases and genes to PARP Antibody (NBP1-50751). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PARP Antibody (NBP1-50751)

Discover more about diseases related to PARP Antibody (NBP1-50751).

Pathways for PARP Antibody (NBP1-50751)

View related products by pathway.

PTMs for PARP Antibody (NBP1-50751)

Learn more about PTMs related to PARP Antibody (NBP1-50751).

Research Areas for PARP Antibody (NBP1-50751)

Find related products by research area.

Blogs on PARP.

The role of PARP-1 in the repair of single stranded break (SSB)
PARPs (poly ADP ribose polymerases) are DNA repair enzymes that promote single stranded break (SSB) repair by binding to DNA at the sites of SSBs and recruiting repair machinery. In humans, the PARP superfamily consists of 17 members, of which five...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PARP1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-50751 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought