PARP [p Asp214] Antibody

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Immunoprecipitation: PARP [p Asp214] Antibody [NBP1-50751] - Immunoprecipitation of HEK293 cells treated with etoposide (5h). Image from verified customer review.

Product Details

Summary
Product Discontinued
View other related PARP Primary Antibodies

Order Details


    • Catalog Number
      NBP1-50751
    • Availability
      Product Discontinued

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PARP [p Asp214] Antibody Summary

Immunogen
Synthetic peptide corresponding to the C-terminus surrounding Asp214 of rat PARP
Modification
p Asp214
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 10-20 ug/ml
  • Immunoprecipitation
  • Immunocytochemistry 10 - 20 ug/ml
Application Notes
The polyclonal anti-cleaved PARP antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP. IP data from customer review.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-50751 in the following applications:

Reactivity Notes

Human reactivity from customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 1.0% BSA, and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PARP [p Asp214] Antibody

  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)
  • ADPRT 1
  • ADPRT
  • ADPRTADP-ribosyltransferase NAD(+)
  • EC 2.4.2
  • EC 2.4.2.30
  • NAD(+) ADP-ribosyltransferase 1
  • PARP apoptosis
  • PARP
  • PARP1
  • PARP-1
  • PARPADPRT1
  • poly (ADP-ribose) polymerase 1
  • poly (ADP-ribose) polymerase family, member 1
  • poly [ADP-ribose] polymerase 1
  • poly(ADP-ribose) polymerase
  • poly(ADP-ribose) synthetase
  • poly(ADP-ribosyl)transferase
  • Poly[ADP-ribose] synthase 1
  • PPOL
  • PPOLpADPRT-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PARP Antibody (NBP1-50751) (0)

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Review for PARP Antibody (NBP1-50751) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using NBP1-50751:
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Images Ratings Applications Species Date Details
Immunoprecipitation PARP NBP1-50751
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reviewed by:
Anonymous
IP Human 08/26/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedHEK293 cells treated with etoposide (5h)
SpeciesHuman
CommentsThere is some background but the signal is pretty consistent.

Blocking

Blocking DetailsNon-fat dry milk (5%) in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 dilution, 3 hrs at RT in 5% non-fat dry milk

Secondary Antibody

Secondary Descriptionanti rabbit HRP-conjugated antibody
Secondary Manufacturer Cat#(Life Technologies # 65-6120)
Secondary Concentration1:20,000

Details

Detection NotesSuperSignal West Pico Chemiluminescent Substrate (Thermo), 2 min exposure using X-ray film
Immunoprecipitation DetailsProtein G PLUS-Agarose (Santa Cruz), 20ul, 4h at 4 degree, washed thrice with PBS containing 0.1% Triton X-100
Elution DescriptionEluted by boiling in 1X SDS dye for 10 minutes

Comments

CommentsThere is some background but the signal is pretty consistent.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PARP Antibody (NBP1-50751). (Showing 1 - 2 of 2 FAQ).

  1. I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
    • For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
  2. Do you have any 100 coda or bigger controls?
    • I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.

Secondary Antibodies

 

Isotype Controls

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Research Areas for PARP Antibody (NBP1-50751)

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Blogs on PARP.

The role of PARP-1 in the repair of single stranded break (SSB)
PARPs (poly ADP ribose polymerases) are DNA repair enzymes that promote single stranded break (SSB) repair by binding to DNA at the sites of SSBs and recruiting repair machinery. In humans, the PARP superfamily consists of 17 members, of which five...  Read full blog post.

Caspase 3 - a Reliable Marker for Index of Apoptosis Induction
Caspase-3 is one of the most important players in apoptosis signaling. It is synthesized as an inactive 32 kDa pro-enzyme and upon direct activation by Caspase-8, -9 or -10, it gets processed into its active forms, the p17-20 and p10-12 subunits. T...  Read full blog post.

Caspase 7 - A key effector of the apoptotic pathway
Caspase-7 is an effector caspase with important roles in mediating cell death signaling. As an effector caspase, caspase-7 is cleaved and activated by initiator caspases such as caspase-1 (1). Like other caspase family proteins, caspase-7 contains a...  Read full blog post.

Caspase 7: The Cell's Suicide Switch
Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all...  Read full blog post.

PARP Antibody Assays aid both Apoptosis and Cancer Research
The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play...  Read full blog post.

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Recent Reviews

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Anonymous
08/26/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol PARP1
Entrez
Uniprot