Western Blot: PARP Antibody [NBP1-31321] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody. HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: PARP Antibody [NBP1-31321] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: PARP Antibody [NBP1-31321] - Analysis of PARP Antibody on: 1: whole cell lysate 2: cytosol extract 3: nuclear extract 4: membrane extract 7.5% SDS PAGE diluted at 1:5000
Western Blot: PARP Antibody [NBP1-31321] - Sample (30 ug of whole cell lysate) A: 293T B: HeLa 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: PARP Antibody [NBP1-31321] - A. 30 ug HCT116 whole cell lysate/extract (untreated). B. 30 ug HCT116 whole cell lysate/extract (30uM cisplatin treatment for 24hr) 7. 5 % SDS-PAGE gel.
Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa and 85 kDa (2, 4).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for PARP Antibody (NBP1-31321). (Showing 1 - 2 of 2 FAQs).
I was wondering if you would mind letting me know which product would you recommend for immunochemistry of the retina of the rat and mouse by poly (ADP-ribose) polymerase (PARP) and calpain. There are many different calpain antibodies in your website. The samples are 12-um vertical cryostst sections (fixed by PFA).
For PARP, I would recommend either NB120-2168 or NB100-64828. Bear in mind that they are both mouse monoclonals, and you may have to take extra steps to reduce mouse-on-mouse background.
Do you have any 100 coda or bigger controls?
I can recommend a couple of high weight antibodies that are conserved in most samples. Please verify the presence of the protein before using as a loading control. PARP1 or PARP is approximately 113kDa, and you may view the products we offer to PARP using this link. Vinculin is approximately 116kDa, and you may view the products we offer to Vinculin using this link.
Caspase 7: The Cell's Suicide Switch Caspase 7 (also known as CASP7, Mch3, ICE-LAP3, CMH-1) is a member of caspase family of cysteine proteases. It is an apoptosis-related cystein peptidase encoded by the CASP7 gene in humans. CASP7 homologous sequences have been identified in nearly all... Read full blog post.
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