Park7/DJ-1 Antibody

Images

 
Western Blot: Park7/DJ-1 Antibody [NB100-483] - Analysis of PARK7 (DJ-1) in wild-type and DJ-1 knockout mice antibody at 1:1,000 dilution. Beta actin is shown as control.
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB100-483] - Park7(DJ-1) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes in Pick's disease. 25 um section.
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes adjacent to brain infarction. 25 um section.

Product Details

Summary
Product Discontinued
View other related Park7/DJ-1 Primary Antibodies
     

Genetic Strategies

   

Order Details


    • Catalog Number
      NB100-483
    • Availability
      Product Discontinued

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Park7/DJ-1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Park7 protein (between residues 100-189) [UniProt Q99497]
Localization
Membrane
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARK7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
Application Notes
For immunohistochemistry, antigen retrieval (boiling in 10mM citrate buffer) is recommended. The standard Western performance in human HepG2 and Jurkat lysates reveals a single band migrating just above the 22kD marker. However, overexposure of blots does reveal cross-reactive bands with higher molecular weight, which do not interfere with the interpretation of standard Westerns. There is sometimes a lower migrating band at approximately 16kDa, but only in the insoluble fraction, both in wild-type and knockout mice. This band is a true non-specific band, but in a fraction that is not containing much DJ-1 anyway. Use in immunprecipitation reported in scientific literature (PMID 21349902) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59657)
Park7/DJ-1 Lysate (NBL1-14106)
Publications
Read Publications using
NB100-483 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Predicted to react with monkey based on 100% sequence homology. Immunogen has 86% homology to chicken and pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

Park7(DJ-1) (Parkinson disease protein 7/ oncogene DJ1, also known as CAP1) was initially discovered as an oncogene promoting Ras-dependent transformation and later it was identified as being responsible for an autosomal recessive early-onset form of Parkinson's disease type 7 (PARK7). Member of peptidase C56 family, Park7(DJ-1) exists as homodimer located predominantly in the cytoplasm to a lesser extent in nucleus as well as mitochondrion, and besides being an essential part of a ternary complex containing PARK7, EFCAB6/DJBP and AR, it interacts with OTUD7B, BBS1, HIPK1, CLCF1, MTERF, EFCAB6/DJBP and PIAS2. Park7(DJ-1) is an evolutionary conserved ubiquitously expressed multifunctional protein involved in several biological processes including transcriptional regulation, oxidative stress, carcinogenesis, cell growth and transformation, AR-dependent transcription regulation, apoptosis, inflammation, redox-sensitive chaperon activities, protein degradation, fertilization and regulation of mitochondrial morphology /function as well as for autophagy of dysfunctional mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Park7/DJ-1 Antibody (NB100-483)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Park7/DJ-1 Antibody (NB100-483) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Bioinformatics

Gene Symbol PARK7
Entrez
Uniprot