Park7/DJ-1 Antibody


Genetic Strategies: Western Blot: Park7/DJ-1 Antibody [NB100-483] - Analysis of PARK7 (DJ-1) in wild-type and DJ-1 knockout mice antibody at 1:1,000 dilution. Beta actin is shown as control.
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB100-483] - Park7(DJ-1) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes in Pick's disease. 25 um section.
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes adjacent to brain infarction. 25 um section.

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Park7/DJ-1 Antibody Summary

A synthetic peptide made to a C-terminal portion of the human Park7 protein (between residues 100-189) [UniProt Q99497]
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation
Application Notes
For immunohistochemistry, antigen retrieval (boiling in 10mM citrate buffer) is recommended. The standard Western performance in human HepG2 and Jurkat lysates reveals a single band migrating just above the 22kD marker. However, overexposure of blots does reveal cross-reactive bands with higher molecular weight, which do not interfere with the interpretation of standard Westerns. There is sometimes a lower migrating band at approximately 16kDa, but only in the insoluble fraction, both in wild-type and knockout mice. This band is a true non-specific band, but in a fraction that is not containing much DJ-1 anyway. Use in immunprecipitation reported in scientific literature (PMID 21349902) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59657)
Park7/DJ-1 Lysate (NBL1-14106)
Park7/DJ-1 Lysate (NBP2-65683)
Read Publications using
NB100-483 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Predicted to react with monkey based on 100% sequence homology. Immunogen has 86% homology to chicken and pig.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for Park7/DJ-1 Antibody

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1


Park7(DJ-1) (Parkinson disease protein 7/ oncogene DJ1, also known as CAP1) was initially discovered as an oncogene promoting Ras-dependent transformation and later it was identified as being responsible for an autosomal recessive early-onset form of Parkinson's disease type 7 (PARK7). Member of peptidase C56 family, Park7(DJ-1) exists as homodimer located predominantly in the cytoplasm to a lesser extent in nucleus as well as mitochondrion, and besides being an essential part of a ternary complex containing PARK7, EFCAB6/DJBP and AR, it interacts with OTUD7B, BBS1, HIPK1, CLCF1, MTERF, EFCAB6/DJBP and PIAS2. Park7(DJ-1) is an evolutionary conserved ubiquitously expressed multifunctional protein involved in several biological processes including transcriptional regulation, oxidative stress, carcinogenesis, cell growth and transformation, AR-dependent transcription regulation, apoptosis, inflammation, redox-sensitive chaperon activities, protein degradation, fertilization and regulation of mitochondrial morphology /function as well as for autophagy of dysfunctional mitochondria.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Park7/DJ-1 Antibody (NB100-483)(5)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Park7/DJ-1 Antibody (NB100-483) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Park7/DJ-1.

Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.

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Gene Symbol PARK7