Genetic Strategies: Western Blot: Park7/DJ-1 Antibody [NB100-483] - Analysis of PARK7 (DJ-1) in wild-type and DJ-1 knockout mice antibody at 1:1,000 dilution. Beta actin is shown as control.
Immunocytochemistry/ Immunofluorescence: Park7/DJ-1 Antibody [NB100-483] - Park7(DJ-1) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes in Pick's disease. 25 um section.
Immunohistochemistry: Park7/DJ-1 Antibody [NB100-483] - Labeling of reactive human astrocytes adjacent to brain infarction. 25 um section.
For immunohistochemistry, antigen retrieval (boiling in 10mM citrate buffer) is recommended. The standard Western performance in human HepG2 and Jurkat lysates reveals a single band migrating just above the 22kD marker. However, overexposure of blots does reveal cross-reactive bands with higher molecular weight, which do not interfere with the interpretation of standard Westerns. There is sometimes a lower migrating band at approximately 16kDa, but only in the insoluble fraction, both in wild-type and knockout mice. This band is a true non-specific band, but in a fraction that is not containing much DJ-1 anyway. Use in immunprecipitation reported in scientific literature (PMID 21349902) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted to react with monkey based on 100% sequence homology. Immunogen has 86% homology to chicken and pig.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Park7/DJ-1 Antibody
Parkinson disease (autosomal recessive, early onset) 7
Parkinson disease protein 7
Park7(DJ-1) (Parkinson disease protein 7/ oncogene DJ1, also known as CAP1) was initially discovered as an oncogene promoting Ras-dependent transformation and later it was identified as being responsible for an autosomal recessive early-onset form of Parkinson's disease type 7 (PARK7). Member of peptidase C56 family, Park7(DJ-1) exists as homodimer located predominantly in the cytoplasm to a lesser extent in nucleus as well as mitochondrion, and besides being an essential part of a ternary complex containing PARK7, EFCAB6/DJBP and AR, it interacts with OTUD7B, BBS1, HIPK1, CLCF1, MTERF, EFCAB6/DJBP and PIAS2. Park7(DJ-1) is an evolutionary conserved ubiquitously expressed multifunctional protein involved in several biological processes including transcriptional regulation, oxidative stress, carcinogenesis, cell growth and transformation, AR-dependent transcription regulation, apoptosis, inflammation, redox-sensitive chaperon activities, protein degradation, fertilization and regulation of mitochondrial morphology /function as well as for autophagy of dysfunctional mitochondria.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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