Papillomavirus 16 E7 Antibody (TVG 701Y)

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Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
TVG 701Y
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Papillomavirus 16 E7 Antibody (TVG 701Y) Summary

Immunogen
HPV 16 E7 synthesised from S. pombe
Localization
Nuclear
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • CyTOF-ready
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human papilloma virus

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Papillomavirus 16 E7 Antibody (TVG 701Y)

  • E7
  • HPV16 E7
  • HpV16gp2
  • Links Transforming protein human papillomavirus 16
  • Protein E7
  • Transforming protein human papillomavirus 16
  • Transforming protein

Background

The HPV E7 proteins are small (HPV16 E7 comprising 98 amino acids, 15kDa), zinc binding phosphoproteins which are localised in the nucleus. They are structurally and functionally similar to the E1A protein of subgenus C adenoviruses. The first 16 amino-terminal amino acids of HPV16 E7 contain a region homologous to a segment of the conserved region 1 (CR1) of the E1A protein of subgenus C adenoviruses. The next domain, up to amino acid 37, is homologous to the entire region 2 (CR2) of E1A. Genetic studies have established that these domains are required for cell transformation in vitro, suggesting similarities in the mechanism of transformation by these viruses. The CR2 homology region contains the LXCXE motif (residues 22-26) involved in binding to the tumour suppressor protein pRb. This sequence is also present in SV40 and polyoma large T antigens. The high risk HPV E7 proteins (of, for example, types 16 and 18) have an approximately ten-fold higher affinity for pRb protein than the low risk HPV E7 proteins (of, for example, type 6). Association of the E7 protein with pRb promotes cell proliferation by the same mechanism as the E1A proteins of adenoviruses and SV40 large T antigen. Recent studies have shown that E7 promotes degradation of Rb family proteins rather than simply inhibiting their function by complex formation. The CR2 region also contains the casein kinase II phosphorylation site (residues 31 and 32). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Papillomavirus 16 E7 Antibody (NB100-2768). (Showing 1 - 1 of 1 FAQ).

  1. Can you tell me the epitope your Papillomavirus 16 E7 Antibody (TVG 701Y) binds to? I'm interested in a monoclonal antibody that binds near to the C terminus (if possible).
    • In regards to your inquiry about our Papillomavirus 16 E7 antibody, unfortunately, we would not be able to provide epitope information. This antibody is raised against the full length protein and we generally do not epitope map our antibodies so we cannot say for sure which location it is binding to. I am sorry that I do not have more specific information at this time.

Secondary Antibodies

 

Isotype Controls

Additional Papillomavirus 16 E7 Products

Papillomavirus 16 E7 NB100-2768

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