pan Cytokeratin Antibody (C-11)

Images

 
Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (C-11) [NBP1-48348] - Analysis using the PE conjugate of NBP1-48348. Staining of cytokeratin on guinea pig breast carcinoma.
Flow (Intracellular): pan Cytokeratin Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348AF647 (blue) and a matched isotype control ...read more
Flow (Intracellular): pan Cytokeratin Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin (C-11) antibody NBP1-48348 (blue) and a matched isotype control NBP2-27287 ...read more
Flow Cytometry: pan Cytokeratin Antibody (C-11) [NBP1-48348] - An intracellular stain was performed on HeLa cells with pan Cytokeratin antibody (C-11) NBP1-48348 (blue) and a matched isotype control NBP2-27287 (orange). ...read more
Flow Cytometry: pan Cytokeratin Antibody (C-11) [NBP1-48348] - Intracellular flow cytometry analysis of cytokeratin expression in HT-29 human Caucasian colon adenocarcinoma cell line using anti-cytokeratin antibody ...read more
Flow Cytometry: pan Cytokeratin Antibody (C-11) [NBP1-48348] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa cells.
Flow Cytometry: pan Cytokeratin Antibody (C-11) [NBP1-48348] - Intracellular flow cytometry analysis of cytokeratin expression in HeLa cells using the APC conjugate.

Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
C-11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

pan Cytokeratin Antibody (C-11) Summary

Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Specificity
This Mouse monoclonal Cytokeratin (Pan-reactive) antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
  • Flow (Intracellular) 1 ug/mL
Application Notes
This antibody is useful in Western Blot, Flow Cytometry, Immunoprecipitation, Immunohistochemistry-paraffin and Immunocytochemistry.
Publications
Read Publications using
NBP1-48348 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Background

CK1 (also designated Cytokeratin 1 or KRT1 ),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pan Cytokeratin Antibody (NBP1-48348)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48348AF405
Alexa Fluor 488 Labeled NBP1-48348AF488
Alexa Fluor 647 Labeled NBP1-48348AF647
Alexa Fluor 700 Labeled NBP1-48348AF700
Allophycocyanin Labeled NBP1-48348APC
DyLight 350 Labeled NBP1-48348UV
DyLight 405 Labeled NBP1-48348V
DyLight 488 Labeled NBP1-48348G
DyLight 680 Labeled NBP1-48348FR
DyLight 755 Labeled NBP1-48348IR
PE Labeled NBP1-48348PE
PerCP Labeled NBP1-48348PCP

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pan Cytokeratin NBP1-48348

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Bioinformatics

Gene Symbol KRT1