PAK5 Overexpression Lysate (Native)

Images

 
Western Blot: PAK5 Overexpression Lysate (Adult Normal) [NBL1-14084] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAK5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PAK5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC214173 and based on accession number NM_177990. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 2, mRNA.
Gene
PAK7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PAK5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PAK5 Overexpression Lysate (Native)

  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA1264MGC26232
  • p21 protein (Cdc42/Rac)-activated kinase 7
  • p21(CDKN1A)-activated kinase 7
  • p21-activated kinase 5
  • p21-activated kinase 7
  • PAK-5
  • PAK5p21CDKN1A-activated kinase 7
  • PAK-7
  • protein kinase PAK5
  • serine/threonine-protein kinase PAK 7
  • serine/threonine-protein kinase PAK7

Background

The protein encoded by this gene is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Both variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PAK5 Lysate (NBL1-14084) (0)

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Bioinformatics

Gene Symbol PAK7