PAK4+5+6 Antibody

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Western Blot: PAK4+5+6 Antibody [NBP1-00971] - Analysis of PAK4/5/6 (V469) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate
Western Blot: PAK4+5+6 Antibody [NBP1-00971] - Western blot (WB) analysis of PAK4+5+6 (V469) antibody in extracts from K562 cell treated with PMA 125ng/ml 30'
Western Blot: PAK4+5+6 Antibody [NBP1-00971] - Analysis of PAK4/5/6 (V469) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Heart tissue lysate of Mouse(40ug) ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00971
    • Availability
      Product Discontinued

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PAK4+5+6 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.
Specificity
PAK4+5+6 (V469) detects endogenous levels of PAK4/5/6 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
PAK4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western blot (WB) analysis of PAK4+5+6 (V469) pAb in extracts from K562 cell treated with PMA 125ng/ml 30'.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAK4+5+6 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA1142
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • p21(CDKN1A)-activated kinase 4
  • p21-activated kinase 4
  • PAK-4
  • protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
  • serine/threonine-protein kinase PAK 4

Background

Three recently identified isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiaekinase STE20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is MEK kinase, an upstream effector of MEK4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP-dependent manner with Rac1 and Cdc42, they do not interact with Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAK4
Entrez