PAK2 Antibody


Western Blot: PAK2 Antibody [NBP1-51318] - Extracts from PC12 cells.
Immunohistochemistry-Paraffin: PAK2 Antibody [NBP1-51318] Human heart tissue.

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PAK2 Antibody Summary

Synthetic peptide, corresponding to amino acids 150-200 of Human PAKgamma.
PAKgamma (R186) pAb detects endogenous levels of PAKgamma protein.
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PAKgamma (R186) pAb in extracts from PC12 cells.
Immunohistochemistry (IHC) analyzes of PAKgamma (R186) pAb in paraffin-embedded human heart tissue.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PAK2 Lysate (NBP2-64668)

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
15mM Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for PAK2 Antibody

  • EC 2.7.11
  • gamma-PAK
  • p21 (CDKN1A)-activated kinase 2
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • p21-activated kinase 2
  • p58
  • PAK2
  • PAK-2
  • PAK65
  • PAK65EC
  • PAKgamma
  • S6/H4 Kinase
  • serine/threonine-protein kinase PAK 2


Three isoforms of serine/threonine kinases, designated 7PAK p68, 8PAK p65 and 9PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The 7, 8, and 9 PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on 9 PAK, including Ser 19, Ser 141 Thr 402, phosphorylation of Ser 141 and Thr 402 is correlated with 9 PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4, which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP dependent manner with Rac 1 and Cdc 42, they do not interact with Rho.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol PAK2