PAK1/2/3 Antibody

Images

 
Western Blot: PAK1/2/3 Antibody [NB100-92201] - analysis of PAK1 (I206) antibody in extracts from 293 cells treated with Etoposide (25uM for 60 min).
Immunohistochemistry: PAK1/2/3 Antibody [NB100-92201] - Immunohistochemistry (IHC) analyzes of PAK1/2/3 (E417/396/415) antibody in paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92201
    • Availability
      Product Discontinued

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PAK1/2/3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 400-450 of Human PAK1.
Clonality
Polyclonal
Host
Rabbit
Gene
PAK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of PAK1 (I206) pAb in extracts from 293 cells treated with Etoposide 25uM 60'.
Immunohistochemistry (IHC) analyzes of PAK1/2/3 (E417/396/415) pAb in paraffin-embedded human brain tissue.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAK1/2/3 Antibody

  • alpha-PAK
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC130000
  • MGC130001
  • p21 protein (Cdc42/Rac)-activated kinase 1
  • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
  • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
  • p21-activated kinase 1
  • p65-PAK
  • PAK-1
  • PAKalpha
  • serine/threonine-protein kinase PAK 1
  • STE20 homolog, yeast

Background

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTPdependent manner with Rac1 and Cdc42, they do not interact with Rho.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Additional PAK1/2/3 Products

PAK1/2/3 NB100-92201

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Bioinformatics

Gene Symbol PAK1
Entrez