PABP Antibody

Images

 
Western Blot: PABP Antibody [NBP1-31840] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: PABP Antibody [NBP1-31840] - Paraffin-embedded mouse lung. PABP antibody [N1N2], N-term dilution: 1:500.
Immunohistochemistry-Paraffin: PABP Antibody [NBP1-31840] - Paraffin-embedded mouse duodenum. PABP antibody [N1N2], N-term dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, MkSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.95 mg/ml

Order Details

PABP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PABP. The exact sequence is proprietary.
Localization
Cytoplasm/Nucleus
Predicted Species
Rat (100%), Monkey (100%), Chicken (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PABPC1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
5.95 mg/ml
Purity
Protein A purified

Alternate Names for PABP Antibody

  • PAB1polyadenylate-binding protein 1
  • PABP
  • PABP-1
  • PABP1poly(A) binding protein, cytoplasmic 2
  • PABPC2
  • PABPL1
  • poly(A) binding protein, cytoplasmic 1
  • Poly(A)-binding protein 1
  • poly(A)-binding protein, cytoplasmic 2

Background

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PABP Antibody (NBP1-31840)

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Bioinformatics

Gene Symbol PABPC1