p97/VCP Antibody

Images

 
Western Blot: p97/VCP Antibody [NBP1-51337] - Extracts from Hela cells and Raw264.7 cells treated with LPS.
Immunohistochemistry-Paraffin: p97/VCP Antibody [NBP1-51337] - Analyzes of VCP (A346) pAb in paraffin-embedded human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51337
    • Availability
      Product Discontinued

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p97/VCP Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 320-370 of Human VCP.
Specificity
VCP (A346) pAb detects endogenous levels of VCP protein.
Clonality
Polyclonal
Host
Rabbit
Gene
VCP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of VCP (A346) pAb in extracts from Hela cells and Raw264.7 cells treated with LPS.
Immunohistochemistry (IHC) analyzes of VCP (A346) pAb in paraffin-embedded human brain tissue.
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p97/VCP Antibody

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VCP
Entrez