p70 S6 Kinase/S6K [p Thr444] Antibody

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Western Blot: p70 S6 Kinase/S6K [p Thr444] Antibody [NBP1-51460] - Analysis of p-p70 S6K (T421) pAb at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with ...read more
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Thr444] Antibody [NBP1-51460] - Analyzes of p-p70 S6 kinase alpha (T421) Antibody in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. ...read more
Western Blot: p70 S6 Kinase/S6K [p Thr444] Antibody [NBP1-51460] - Analyzes of p-p70 S6 kinase Alpha (S434) pAb in extracts from NIH/3T3 EGF cells.
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Thr444] Antibody [NBP1-51460] - Analyzes of p-p70 S6 kinase Alpha (T444) pAb in paraffin-embedded human breast carcinoma tissue.
Immunohistochemistry-Paraffin: p70 S6 Kinase/S6K [p Thr444] Antibody [NBP1-51460] - Analyzes of p-p70 S6 kinase alpha (T421) Antibody in paraffin-embedded human breast carcinoma tissue at 1:50.showing nucleus staining. ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51460
    • Availability
      Product Discontinued

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p70 S6 Kinase/S6K [p Thr444] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human p70 S6K around the phosphorylation site of Threonine 421.
Modification
p Thr444
Specificity
p-p70 S6 kinase alpha (T444) pAb detects endogenous levels of p70 S6 kinase alpha protein only when phosphorylated at T444.
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of p-p70 S6 kinase alpha (S434) pAb in extracts from NIH/3T3 EGF cells.
Immunohistochemistry (IHC) analyzes of p-p70 S6 kinase alpha (T444) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K [p Thr444] Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

In studies to elucidate key regulatory pathways in signal transduction, several protein serine/threonine (Ser/Thr) kinases have been identified. Included among such kinases are two distinct families of 40S Ribosomal Protein S6 Ser/Thr kinases present in somatic animal cells, designated p70 S6 kinase and p90 Rsk kinase. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathway that regulate these kinases. In fact, evidence suggests that one such family of activating enzymes includes the members of the ERK MAP kinase family. The ERK MAP kinases are, in turn, regulated by phosphorylation at threonine and tyrosine residues by a protein kinase designated MEK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on p70 S6 Kinase/S6K.

S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.

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Bioinformatics

Gene Symbol RPS6KB1
Entrez