p70 S6 Kinase/S6K Antibody (6B2)

Images

 
Western Blot: S6K Antibody (6B2) [NBP2-00770] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: S6K Antibody (6B2) [NBP2-00770] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY S6K.
Immunohistochemistry-Paraffin: S6K Antibody (6B2) [NBP2-00770] - Staining of paraffin-embedded Human tonsil using anti-S6K mouse monoclonal antibody.
Western Blot: S6K Antibody (6B2) [NBP2-00770] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S6K (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug ...read more

Product Details

Summary
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Order Details


    • Catalog Number
      NBP2-00770
    • Availability
      Product Discontinued

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p70 S6 Kinase/S6K Antibody (6B2) Summary

Immunogen
Full length human recombinant protein of human RPS6KB1(NP_003152) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPS6KB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Reviewed Applications
Read 1 Review rated 5
using
NBP2-00770 in the following applications:

Reactivity Notes

Human, Dog, Monkey, Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K Antibody (6B2)

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p70 S6 Kinase/S6K Antibody (NBP2-00770) (0)

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Review for p70 S6 Kinase/S6K Antibody (NBP2-00770) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-00770:
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Images Ratings Applications Species Date Details
Western Blot p70 S6 Kinase/S6K NBP2-00770
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reviewed by:
Anonymous
WB Human 08/16/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p70 S6 Kinase/S6K Antibody (NBP2-00770) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for p70 S6 Kinase/S6K Antibody (NBP2-00770)

Discover related pathways, diseases and genes to p70 S6 Kinase/S6K Antibody (NBP2-00770). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for p70 S6 Kinase/S6K Antibody (NBP2-00770)

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Research Areas for p70 S6 Kinase/S6K Antibody (NBP2-00770)

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Blogs on p70 S6 Kinase/S6K.

S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.

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Recent Reviews

5
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1
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Anonymous
08/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RPS6KB1
Entrez