p53 Antibody (D01)

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Western Blot: p53 Antibody (D01) [NBP1-41199] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01]. HRP-conjugated ...read more
Chromatin Immunoprecipitation: p53 Antibody (D01) [NBP1-41199] - HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr. A. 5 ug preimmune mouse IgG. B. 5 ug of p53 antibody. The precipitated DNA was detected ...read more
Western Blot: p53 Antibody (D01) [NBP1-41199] - Detection of human p53 protein using p53 DO1 monoclonal antibody in HBL100 whole cell extract.
Western Blot: p53 Antibody (D01) [NBP1-41199] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ...read more
Western Blot: p53 Antibody (D01) [NBP1-41199] - Sample (30 ug of whole cell lysate) A: 293T B: A431 10% SDS PAGE; antibody diluted at 1:10000.
Western Blot: p53 Antibody (D01) [NBP1-41199] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:5000.
Western Blot: p53 Antibody (D01) [NBP1-41199] - Sample (30 ug of whole cell lysate) A: U2OS cells with mock treatment for 24hr B: U2OS cells with 30uM cisplatin treatment for 24hr 10% SDS PAGE; antibody diluted at ...read more
Western Blot: p53 Antibody (D01) [NBP1-41199] - Non-transfected (-) and transfected (+) HCT116 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01]. ...read more
Western Blot: p53 Antibody (D01) [NBP1-41199] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [D01]. HRP-conjugated ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-41199
    • Availability
      Product Discontinued

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p53 Antibody (D01) Summary

Immunogen
Recombinant human p53 expressed in E. coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TP53
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p53 Antibody (D01)

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

p53-DO1 specifically recognizes an epitope at the N-terminal end of the human p53 protein between amino acids 20-25. p53 is a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. And p53, as a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15, a mutation which reduces the ability of p53 to arrest cell growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p53 Antibody (NBP1-41199) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for p53 Antibody (NBP1-41199)

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Research Areas for p53 Antibody (NBP1-41199)

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Blogs on p53. Showing 1-10 of 16 blog posts - Show all blog posts.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

p14 ARF is an Important Tumor Suppressor
The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TP53
Entrez
Uniprot