p40 (p63 delta) Antibody

Images

 
Western Blot: RPSA Antibody [NBP1-32956] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RPSA Antibody [NBP1-32956] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-32956] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
Immunoprecipitation: p40 (p63 delta) Antibody [NBP1-32956] - Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 ug of p40 antibody [N3C3]. Western blot analysis was performed using p40 antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

p40 (p63 delta) Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human p40. The exact sequence is proprietary.
Localization
Cytoplasm, Endosome membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RABEPK
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p40 (p63 delta) Antibody

  • 40 kDa Rab9 effector protein
  • bA65N13.1
  • p40
  • p40-p63 delta
  • Rab9 effector p40
  • Rab9 effector protein with kelch motifs
  • RAB9P40DKFZp686P1077
  • RABEPK

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RABEPK