p38 [p Thr180, p Tyr182] Antibody

Images

 
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Hela cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the -39 kDa p38 MAPK protein phosphorylated at ...read more
Immunohistochemistry-Paraffin: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Immunostaining of human breast cancer tissue showing p38 when phosphorylated at Thr180/Tyr182 in red.
Western Blot: p38 [p Thr180, p Tyr182] Antibody [NB500-138] - Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Pm, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

p38 [p Thr180, p Tyr182] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr180 and phospho-Tyr182 of rat p38 MAPK.
Modification
p Thr180, p Tyr182
Localization
Cytoplasmic and Nuclear.
Specificity
Specific for the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%), Chicken (100%), Canine (100%), Zebrafish (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB500-138 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for p38 [p Thr180, p Tyr182] Antibody

  • CSAID-binding protein
  • Csaids binding protein
  • CSBP
  • CSBP1MAX-interacting protein 2
  • CSBP2
  • CSPB1
  • cytokine suppressive inflammatory drug binding protein
  • Cytokine suppressive inflammatory drug-binding protein
  • EC 2.7.11
  • EC 2.7.11.24
  • EXIP
  • MAP kinase 14
  • MAP kinase MXI2
  • MAP kinase p38 alpha
  • MAPK 14
  • mitogen-activated protein kinase 14
  • Mitogen-activated protein kinase p38 alpha
  • Mxi2
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • p38
  • p38alpha Exip
  • p38ALPHA
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • stress-activated protein kinase 2A

Background

P38 is a reactivating kinase that is a member of the MAP kinase family. It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress. It is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003; Ying et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P

Publications for p38 Antibody (NB500-138)(3)

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Blogs on p38.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK14
Entrez
Uniprot