p38 Antibody

Images

 
Western Blot: p38 Antibody [NB110-96907] - analysis of Human cancer cell lines showing detection of p38 protein using Rabbit Anti-p38 Polyclonal Antibody. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. ...read more
Immunocytochemistry/ Immunofluorescence: p38 Antibody [NB110-96907] - Analysis using Rabbit Anti-p38 Polyclonal Antibody. Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

p38 Antibody Summary

Immunogen
Protein corresponding to the molecular mass of p38 on SDS PAGE immunoblots.A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH
Localization
Cytoplasm, Nucleus
Specificity
Detects approx 43kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:250
Application Notes
A 1:1000 dilution of p38 Antibody was sufficient for detection of p38 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p38 Lysate (NBL1-12873)

Reactivity Notes

Based on homology, It is predicted to detect Mouse, Rat, Bovine, Monkey, Rabbit, Porcine, Canine, Hamster, Chicken, Sheep and Guinea Pig

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS pH7.4, 50% glycerol.
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p38 Antibody

  • CSAID-binding protein
  • Csaids binding protein
  • CSBP
  • CSBP1MAX-interacting protein 2
  • CSBP2
  • CSPB1
  • cytokine suppressive inflammatory drug binding protein
  • Cytokine suppressive inflammatory drug-binding protein
  • EC 2.7.11
  • EC 2.7.11.24
  • EXIP
  • MAP kinase 14
  • MAP kinase MXI2
  • MAP kinase p38 alpha
  • MAPK 14
  • mitogen-activated protein kinase 14
  • Mitogen-activated protein kinase p38 alpha
  • Mxi2
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • p38
  • p38alpha Exip
  • p38ALPHA
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • stress-activated protein kinase 2A

Background

p38 Antibody Background: The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway. The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2 alpha translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death. Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP. p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs. As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis and has also been implicated in cancer, CNS and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p38 Antibody (NB110-96907) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for p38 Antibody (NB110-96907)

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Blogs on p38.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK14
Entrez
Uniprot