p38 [p Tyr182] Antibody

Images

 
Western Blot: p38 [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).
Immunohistochemistry: p38 [p Tyr182] Antibody [NB100-82097] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using P38 MAPK (phospho- Tyr182) antibody
Western Blot: p38 [p Tyr182] Antibody [NB100-82097] - Western blot analysis of extracts from NIH-3T3 (Lanes 1, 4, 7 and 10) and cos7 (Lanes 2, 5, 8 and 11 and K562 (Lanes 3, 6, 9 and 12) cells, untreated or treated with ...read more
Western Blot: p38 [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK(Phospho-Tyr182) antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

p38 [p Tyr182] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).
Modification
p Tyr182
Specificity
p38 MAPK (specific to Phospho-Tyr182) detects endogenous levels of P38MAPK only when phosphorylated at tyrosine 182.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p38 Lysate (NBL1-12873)
Publications
Read Publication using
NB100-82097 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS [pH 7.4] containing 150mM NaCl, and 50% glycerol (without Mg2+ and Ca2+)
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p38 [p Tyr182] Antibody

  • CSAID-binding protein
  • Csaids binding protein
  • CSBP
  • CSBP1MAX-interacting protein 2
  • CSBP2
  • CSPB1
  • cytokine suppressive inflammatory drug binding protein
  • Cytokine suppressive inflammatory drug-binding protein
  • EC 2.7.11
  • EC 2.7.11.24
  • EXIP
  • MAP kinase 14
  • MAP kinase MXI2
  • MAP kinase p38 alpha
  • MAPK 14
  • mitogen-activated protein kinase 14
  • Mitogen-activated protein kinase p38 alpha
  • Mxi2
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • p38
  • p38alpha Exip
  • p38ALPHA
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • stress-activated protein kinase 2A

Background

p38 is a 38 kDa Stress Activated Protein Kinase / Map Kinase (SAPK / MAPK) that is fully activated by dual phosphorylation on threonine 180 and tyrosine 182, within the activation loop. p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2 and the kinases MAPKAPK2 and MAPKAPK5. p38 MAPK plays a role in the production of IL6 and is thought to stabilize erythropoietin production during hypoxic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p38 Antibody (NB100-82097)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on p38.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK14
Entrez
Uniprot