p38 Antibody (8G4D11)

Images

 
Western Blot: p38 Antibody (8G4D11) [NBP2-37378] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MAPK14 mouse mAb with DAB staining.
Immunocytochemistry/ Immunofluorescence: p38 Antibody (8G4D11) [NBP2-37378] - Immunofluorescence analysis of HeLa cells using MAPK14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry: p38 Antibody (8G4D11) [NBP2-37378] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MAPK14 mouse mAb with DAB staining.
Flow Cytometry: p38 Antibody (8G4D11) [NBP2-37378] - Flow cytometric analysis of HeLa cells using MAPK14 mouse mAb (green) and negative control (red).
Western Blot: p38 Antibody (8G4D11) [NBP2-37378] - Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 33 kDa)
Western Blot: p38 Antibody (8G4D11) [NBP2-37378] - Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: p38 Antibody (8G4D11) [NBP2-37378] - Western blot analysis using MAPK14 mouse mAb against HeLa (1), NIH/3T3 (2), Jurkat (3), Raw264.7 (4), PC-12 (5), C6 (6) and COS7 (7) cell lysate.
ELISA: p38 Antibody (8G4D11) [NBP2-37378] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
8G4D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

p38 Antibody (8G4D11) Summary

Immunogen
Purified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAPK14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:75
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • CyTOF-ready
Theoretical MW
41.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for p38 Antibody (8G4D11)

  • CSAID-binding protein
  • Csaids binding protein
  • CSBP
  • CSBP1MAX-interacting protein 2
  • CSBP2
  • CSPB1
  • cytokine suppressive inflammatory drug binding protein
  • Cytokine suppressive inflammatory drug-binding protein
  • EC 2.7.11
  • EC 2.7.11.24
  • EXIP
  • MAP kinase 14
  • MAP kinase MXI2
  • MAP kinase p38 alpha
  • MAPK 14
  • mitogen-activated protein kinase 14
  • Mitogen-activated protein kinase p38 alpha
  • Mxi2
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • p38
  • p38alpha Exip
  • p38ALPHA
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • stress-activated protein kinase 2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on p38.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK14