p27/Kip1 Antibody

Images

 
Western Blot: p27/Kip1 Antibody [NB100-82093] - Analysis using p27Kip1 antibody: Lane1: The extract from A2780 cells; Lane2: The extract from COLO20 degrees Cells; Lane3: The extract from COLO20 degrees Cells using ...read more
Immunohistochemistry: p27/Kip1 Antibody [NB100-82093] - Analysis of paraffin-embedded human lung carcinoma tissue using p27Kip1 (Ab-10) antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

p27/Kip1 Antibody Summary

Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around aa. 10 (N-G-S-P-S).
Specificity
p27Kip1 (specific to a.a. 10) detects endogenous levels of total p27Kip1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500~1:1000
  • Immunohistochemistry 1:50~1:100
  • Immunohistochemistry-Paraffin 1:50~1:100
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
p27/Kip1 Lysate (NBL1-09051)
Publications
Read Publication using
NB100-82093 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p27/Kip1 Antibody

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p27/Kip1 Antibody (NB100-82093)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDKN1B
Entrez
Uniprot