p27/Kip1 Antibody

Images

 
Western Blot: p27/Kip1 Antibody [NB100-78265] - Total cell lysates (15 ug protein) from MCF-7, NIH3T3, and Raw264.7 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with ...read more
Western Blot: p27/Kip1 Antibody [NB100-78265] - Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p27Kip1 antibody. Proteins were visualized using a donkey ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-78265
    • Availability
      Product Discontinued

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p27/Kip1 Antibody Summary

Immunogen
Peptide derived from the carboxy terminus of human p27
Specificity
p27Kip1
Clonality
Polyclonal
Host
Rabbit
Gene
CDKN1B
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Positive Control
p27/Kip1 Lysate (NBL1-09051)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for p27/Kip1 Antibody

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27Kip1 (cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor p27) is a 27kD cyclin-dependent inhibitor. p27Kip1 is localized in the nucleus and cytoplasm (phospho-specific). This protein functions in G1 arrest and binds and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2. p27Kip1 mediates TGF beta-induced G1 arrest and regulates cell motility. This kinase has also been implicated in translational control. Akt phosphorylation causes p27 Kip1 export to cytoplasm; p27 Kip1 has also been shown to be ubiquitinated. p27 Kip1 has been shown to interact with NUP50, Skp2, and PKB/Akt. The Poly6029 antibody recognizes the N-terminal region of human p27Kip1 and has been shown to be useful for Western blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for p27/Kip1 Antibody (NB100-78265)

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Bioinformatics

Gene Symbol CDKN1B
Entrez