p27/Kip1 Antibody (SX53G8) [DyLight 405LS]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SX53G8
Clonality
Monoclonal
Host
Mouse

p27/Kip1 Antibody (SX53G8) [DyLight 405LS] Summary

Immunogen
Purified GST-p27 fusion protein of human origin
Localization
Nuclear
Specificity
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1B
Purity
Protein A purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 405LS
Format
DyLight 405LS (A=397, E=572)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Alternate Names for p27/Kip1 Antibody (SX53G8) [DyLight 405LS]

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p27/Kip1 Antibody (NBP2-34718V3) (0)

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Secondary Antibodies

 

Isotype Controls

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Diseases for p27/Kip1 Antibody (NBP2-34718V3)

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CDKN1B
Entrez

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