p21/CIP1/CDKN1A Antibody (2F1)

Western Blot: p21/CIP1/CDKN1A Antibody (2F1) [H00001026-M02] - CDKN1A monoclonal antibody (M02), clone 2F1. Analysis of CDKN1A expression in human liver.

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (2F1) [H00001026-M02] - Analysis of monoclonal antibody to CDKN1A on HeLa cell . Antibody concentration 10 ug/ml.

Proximity Ligation Assay: p21/CIP1/CDKN1A Antibody (2F1) [H00001026-M02] - Analysis of protein-protein interactions between CASP3 and CDKN1A. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-CDKN1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: p21/CIP1/CDKN1A Antibody (2F1) [H00001026-M02] - Detection limit for recombinant GST tagged CDKN1A is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

p21/CIP1/CDKN1A Antibody (2F1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDKN1A (AAH00312.1, 65 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
• Specificity: CDKN1A - cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDKN1A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for p21/CIP1/CDKN1A Antibody (2F1)

• CAP20cyclin-dependent kinase inhibitor 1
• CDK-interacting protein 1
• CDKN1A
• CDKN1melanoma differentiation associated protein 6
• CIP1
• CIP1WAF1CDK-interaction protein 1
• cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• MDA6
• MDA-6
• Melanoma differentiation-associated protein 6
• p21
• p21CIP1
• p21Cip1/Waf1
• PIC1
• SDI1DNA synthesis inhibitor
• wild-type p53-activated fragment 1

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p21/CIP1/CDKN1A Antibody (H00001026-M02)(2)

Publications using H00001026-M02

• Massimi M, Cardarelli S, Galli F et al. Increase of Intracellular Cyclic Amp by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival. J Cell Biochem 2016-11-16 [PMID: 27859531]
• Huang Y, Chuang A, Hao H et al. Phospho-?GNp63? is a key regulator of the cisplatin-induced microRNAome in cancer cells. Cell Death Differ 2011-01-28 [PMID: 21274007]

Bioinformatics

• Gene Symbol: CDKN1A
• Entrez:
  • 1026(Human)
• OMIM:
  • 116899(Human)
• Uniprot:
  • NP_000380(Human)