p190RhoGAP [p Tyr1105] Antibody

Images

 
Western Blot: p190RhoGAP [p Tyr1105] Antibody [NB100-92688] - Lane1:Hela whole cell lysate. Lane2:Rat brain tissue lysate Lane3:PC12 whole cell lysate.
Immunohistochemistry-Paraffin: p190RhoGAP [p Tyr1105] Antibody [NB100-92688] - Human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92688
    • Availability
      Product Discontinued

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p190RhoGAP [p Tyr1105] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1105.
Modification
p Tyr1105
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGAP35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Immunohistochemistry analysis of p-GRF-1 (Y1105) Antibody in paraffin-embedded human breast carcinoma tissue
Theoretical MW
172 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-92688.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p190RhoGAP [p Tyr1105] Antibody

  • glucocorticoid receptor DNA binding factor 1
  • glucocorticoid receptor DNA-binding factor 1
  • glucocorticoid receptor repression factor 1
  • GRF1
  • GRF-1
  • GRLF1
  • KIAA1722
  • MGC10745
  • P190A
  • P190-A
  • p190ARhoGAP
  • p190RhoGAP
  • rho GAP p190A
  • Rho GTPase activating protein 35
  • rho GTPase-activating protein 35

Background

GRLF1 (glucocorticoid receptor DNA binding factor), is a repressor of glucocorticoid receptor transcription and associates with the promoter region of the glucocorticoid receptor gene (hGR gene). The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. It's level of expression is regulated by glucocorticoids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p190RhoGAP Antibody (NB100-92688)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for p190RhoGAP Antibody (NB100-92688). (Showing 1 - 1 of 1 FAQ).

  1. Is this antibody specific to the phosphorylated p190RhoGAP (as opposed to just reacting with the nonphosphorylated form)?
    • With regard to your request regarding product NB100-92688, the antibody was made to the modified peptide sequence phosphorylated at Tyr 1105. As the product was generated against an immunogen 15 amino acids long and is polyclonal, there is a chance that the antibody will detect both forms. The antibody was purified against the phosphorylated immunogen, but was not cross adsorbed against the non-phosphorylated protein. We do not have any additional testing data for this product at this time.

Secondary Antibodies

 

Isotype Controls

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PTMs for p190RhoGAP Antibody (NB100-92688)

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Bioinformatics

Gene Symbol ARHGAP35
Entrez
Uniprot