OSTF1 Antibody (2F11)

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Western Blot: OSTF1 Antibody (AT2F11) [NBP1-97933] The extracts of Mouse spleen (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 (1:500~1:5000). Proteins were visualized ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
2F11
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

OSTF1 Antibody (2F11) Summary

Immunogen
Recombinant human OSTF1 (1-217aa) purified from E. coli
Specificity
Anti-human OSTF1 mAb, clone 2F11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human OSTF1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OSTF1
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000. Recommended starting dilution is 1:1000

Alternate Names for OSTF1 Antibody (2F11)

  • FLJ20559
  • OSFSH3P2bA235O14.1
  • osteoclast stimulating factor 1
  • osteoclast-stimulating factor 1

Background

Osteoclast-stimulating factor (OSTF1) is an intracellular signaling protein, highly expressed by osteoclasts(OCL). Indirectly, it enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade by possibly interacting with c-Src or other c-Src-related proteins. OSTF1 contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate-rich C-terminal region, suggesting that it is potentially involved in protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

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Bioinformatics

Gene Symbol OSTF1
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