OSCAR Antibody (5B8)

Flow Cytometry: OSCAR Antibody (5B8) [NBP3-11201] - Analysis of OSCAR in RAW cells.

Product Discontinued

OSCAR Antibody (5B8) Summary

• Immunogen: The monoclonal antibody 5B8 reacts with mouse osteoclast-associated receptor (OSCAR).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: OSCAR
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
• Application Notes: The monoclonal antibody can be used for analysis of mouse OSCAR in flow cytometry as well as functional studies for osteoclastogenesis. For Flow Cytometry and Immunocytochemistry/Immunofluorescence, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for OSCAR Antibody (5B8)

• hOSCAR
• MGC33613
• OSCAR
• osteoclast associated receptor OSCAR-S1
• osteoclast associated receptor OSCAR-S2
• osteoclast associated, immunoglobulin-like receptor
• osteoclast-associated immunoglobulin-like receptor
• Osteoclast-associated receptor
• PIGR3
• PIgR-3
• Poly-Ig receptor 3
• Polymeric immunoglobulin receptor 3

Background

The monoclonal antibody 5B8 reacts with mouse osteoclast-associated receptor (OSCAR), which is a 265 amino acid type 1 transmembrane protein belonging to the leukocyte receptor complex protein family that play critical roles in the regulation of both innate and adaptive immune responses. In mice, OSCAR is a RANKL-inducible gene which- is expressed during the later stages of pre-osteoclast maturation and is shown to be a potent FcR and gamma-associated costimulatory receptor in vitro. OSCAR promotes osteoclastogenesis in vivo, and OSCAR binding to its collagen ligand led to signaling that- is involved in functional differentiation of leukocytes. In recent studies human OSCAR has been shown to play multiple roles in the immune system including activation and maturation of immune cells, prevention of apoptosis and enhancement of pro-inflammatory circuits. The latter by- inducing intracellular calcium release resulting in sustained IL-8 and chemokine expression leading to chemotaxis of Th2 effector and regulatory T-cells. In a- murine model using calvarias bone explants, the synovial fluid of osteoarthritis patients resulted in increased OSCAR mRNA expression suggesting that OSCAR might contribute to in the degenerative process. Moreover, in a recent study- OSCAR was found to be- induced in monocytes of RA patients, facilitating their differentiation into osteoclasts and bone resorption. The monoclonal antibody, 5B8, can be used for analysis of mouse OSCAR in flow-cytometry as well as functional studies for osteoclastogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OSCAR Antibody (NBP3-11201)(2)

Publications using NBP3-11201

• Omata Y, Tachibana H, Aizaki Y et al. Essentiality of Nfatc1 short isoform in osteoclast differentiation and its self-regulation Scientific reports 2023-11-01 [PMID: 37914750] (ICC/IF, Mouse)
• Peek CT, Ford CA, Eichelberger KR et al. Intestinal inflammation promotes MDL-1+ osteoclast precursor expansion to trigger osteoclastogenesis and bone loss Cellular and molecular gastroenterology and hepatology 2022-07-11 [PMID: 35835390] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: OSCAR