Recombinant Human Oncostatin M/OSM Protein

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SDS-Page: Human Oncostatin M/OSM 195 a.a Protein [NBP2-35115]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

Recombinant Human Oncostatin M/OSM Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 195 residues of OSM.

Source: E. coli

Amino Acid Sequence: AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEETLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILGLRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSVGRVFSKWGES PNRSR

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
Oncostatin M Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
OSM
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
22.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-35115 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human Oncostatin M/OSM Protein

  • MGC20461
  • oncostatin M
  • oncostatin-M
  • OSM

Background

Oncostatin M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. The effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant human OSM has approximately 40 % and 39 % amino acid sequence identity with rat and murine OSM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Review for Oncostatin M/OSM Protein (NBP2-35115) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-35115:
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 Oncostatin M/OSM NBP2-35115
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5
reviewed by:
Anonymous
01/04/2017
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Summary

CommentsI ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

CommentsSDS PAGE gel with Coomassie blue staining showing detection of Human oncostatin M/OSM Protein (NBP2-35115) (0.5 ug) at about 20 kDa.

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Recent Reviews

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Anonymous
01/04/2017
Application:
Species:

Bioinformatics

Gene Symbol OSM
Uniprot