Olig2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: OLIG2 Antibody [NBP1-46676] - ICC/IC analysis of OLIG2 in mouse oligodendrocytes. Image courtesy of anonymous customer product review.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-46676
    • Availability
      Product Discontinued

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Olig2 Antibody Summary

Immunogen
A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and mouse.
Marker
Oligodendrocyte Marker
Specificity
Specific for OLIG2.
Clonality
Polyclonal
Host
Sheep
Gene
OLIG2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 10-50 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemistry-Paraffin/Frozen, Western Blot. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. ICC/IF - See customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-46676 in the following applications:

Reactivity Notes

Human, rat, mouse. Other species not yet tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized water.

Alternate Names for Olig2 Antibody

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Olig2 Antibody (NBP1-46676) (0)

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Review for Olig2 Antibody (NBP1-46676) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-46676:
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IF
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Olig2 NBP1-46676
Enlarge
reviewed by:
Pia Vidal
IF Mouse 09/03/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary culture of oligodendrocytes
SpeciesMouse
Lot02202012
CommentsAlso tested this antibody in spinal cord cryosections, where the antibody does not work. However, as you can see in the attached image, in fixed cells for this antibody works nicely.

Blocking

Blocking Details10% serum, 0.5% triton and PBS for 1 hour

Primary Anitbody

Dilution Ratio5% serum, 0.3% triton and PBS overnight (4°C). Dilution 1/100

Secondary Antibody

Secondary DescriptionAlexa fluor 555, donkey anti-sheep
Secondary Manufacturer Cat#W3031B
Secondary Concentration500µL

Details

Detection NotesNikon fluorescent microscope. Negative control: Sample without the primary antibody. Exposure time: 11ms
Fixation Details4% PFA for 30 minutes at RT
Wash Description3 washes of 5 minutes with PBS

Comments

CommentsAlso tested this antibody in spinal cord cryosections, where the antibody does not work. However, as you can see in the attached image, in fixed cells for this antibody works nicely.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Olig2 Antibody (NBP1-46676). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in two of your antibodies: OLIG2 (NBP1-30650) and OLIG2 (NBP1-46676), but I could not find information in your webpage regarding the use of either of the two antibodies in cryosection of mouse tissue. Do you have information about it?, because I would like to use them in spinal cord cryosection from mouse.
    • We have tested these antibodies in IHC-Fr and IHC- Paraffin, and they are both specific to mouse.

Secondary Antibodies

 

Isotype Controls

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Blogs on Olig2

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Contact Information

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Calculators

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Recent Reviews

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Pia Vidal
09/03/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol OLIG2
Entrez