Olig2 Antibody

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Western Blot: Olig2 Antibody [NBP1-30649] - Mouse brain lysate (denatured, reduced) using Rabbit antibody to n-terminal region of OLIG2 (Oligo2, bHLHB1, RACK17): IgG at a concentration of 30 ug/ml ; blocked with 1% LFDM ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-30649
    • Availability
      Product Discontinued

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Olig2 Antibody Summary

Immunogen
A synthetic peptide from the n-terminal region of human OLIG2 (Oligo2, bHLHB1, RACK17) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.
Marker
Oligodendrocyte Marker
Specificity
Appears to be specific for OLIG2.
Clonality
Polyclonal
Host
Rabbit
Gene
OLIG2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
Application Notes
WB. A concentration of 10-50 ug/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications. Although not tested, this antibody may work in IHC-Paraffin/Frozen.

Reactivity Notes

Human, Rat, Mouse. Other species not yet tested.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
IgG purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized water.

Alternate Names for Olig2 Antibody

  • BHLHB1
  • BHLHE19
  • Class B basic helix-loop-helix protein 1
  • Class E basic helix-loop-helix protein 19
  • human protein kinase C-binding protein RACK17
  • Olig2
  • OLIGO2
  • oligodendrocyte lineage transcription factor 2
  • oligodendrocyte transcription factor 2
  • oligodendrocyte-specific bHLH transcription factor 2
  • PRKCBP2
  • protein kinase C binding protein 2
  • Protein kinase C-binding protein 2
  • Protein kinase C-binding protein RACK17
  • RACK17
  • RACK17helix-loop-helix protein, class B, 1

Background

Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OLIG2
Entrez