OCT4 Antibody


Western Blot: OCT4 Antibody [NBP1-52037] - (1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

OCT4 Antibody Summary

Peptide with sequence C-DRPNAVKLEKVEP corresponding to internal region according to Uniprot Mouse NP_038661.2.
Embryonic Stem Cell Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
WB: Approx. 40 kDa band observed in NIH3T3 lysates (calculated MW of 38.2 kDa band according to NP_038661.2).
Read Publication using NBP1-52037.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Alternate Names for OCT4 Antibody

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein


Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and a central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct-4 is downregulated thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is nucleus protein, with a predicted molecular weight 39 kD. The poly6318 antibody recognizes human Oct4 and has been shown to be useful for immunofluorescent staining. SOX2, SOX8, SOX10, androgen receptor and ETS2


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OCT4 Antibody (NBP1-52037)(1)

Reviews for OCT4 Antibody (NBP1-52037) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for OCT4 Antibody (NBP1-52037) (0)

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Secondary Antibodies


Isotype Controls

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Blogs on OCT4.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol POU5F1

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