OCT2 Antibody (9A2)

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Western Blot: OCT2 Antibody (9A2) [NBP2-00351] - Ramos cell line lysates prepared under reducing conditions were resolved by SDS-PAGE then immunoblotted with 3 ug/ml of Anti-Human/Mouse OCT2 Purified. Bands were ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-00351
    • Availability
      Product Discontinued

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OCT2 Antibody (9A2) Summary

Immunogen
The immunogen for this antibody is Oct2.
Isotype
IgG2 Kappa
Clonality
Monoclonal
Host
Rat
Gene
POU2F2
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This 9A2 antibody has been tested by western blot analysis of the Ramos cell line under reducing conditions. This can be used at less than or equal to 5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for OCT2 Antibody (9A2)

  • homeobox protein
  • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2
  • OCT2Oct-2
  • Octamer-binding protein 2
  • Octamer-binding transcription factor 2
  • OTF-2
  • OTF2POU domain class 2, transcription factor 2
  • POU class 2 homeobox 2
  • POU domain, class 2, transcription factor 2

Background

This monoclonal 9A2 antibody recognizes human and mouse Oct2, a transcription factor that binds conserved octamer DNA motifs within immunoglobulin promoters. The structure of the Oct2 protein consists of an amino terminal glutamine-rich domain, a central POU homeodomain and a carboxy terminal transactivation domain. Predominantly expressed in the nucleus of B cells, Oct2 has also been found in activated T cells and a subset of myeloid cells. Based on knock-out studies, expression is not required for B cell commitment but is required for maturation. In addition to developmental expression, Oct2 has been associated with neoplasm and maybe a useful tool for characterization of DLBCL and classical Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU2F2