OCT1 Antibody

Images

 
Western Blot: OCT1 Antibody [NBP1-52035] - Analysis of 41913 in nuclear HeLa lysate (35ug protein in RIPA buffer) using NBP1-52035 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

OCT1 Antibody Summary

Immunogen
Peptide with sequence C-NVQSKSNEESGDSQQ corresponding to internal region (near N-Terminus) according to NP_002688.2.
Epitope
C-NVQSKSNEESGDSQQ
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU2F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 100 kDa band observed in HeLa nuclear lysates (calculated MW of 76.5 kDa band according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-52035.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OCT1 Antibody

  • FLJ42836
  • NF-A1
  • Oct-1
  • OCT1class 2, transcription factor 1
  • OTF1
  • POU class 2 homeobox 1
  • POU domain class 2, transcription factor 1
  • POU2F1

Background

Oct1 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR and interacts with NR3C1, AR and PGR. Oct1 is Ubiquitous and belongs to the POU transcription factor family class 2 subfamily. Oct1 contains 1 homeobox domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OCT1 Antibody (NBP1-52035)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for OCT1 Antibody (NBP1-52035)

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Bioinformatics

Gene Symbol POU2F1
Entrez
Uniprot