OBFC1 Antibody (2E4)

Images

 
Western Blot: OBFC1 Antibody (2E4) [NBP2-01006] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: OBFC1 Antibody (2E4) [NBP2-01006] Staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1.
Flow Cytometry: OBFC1 Antibody (2E4) [NBP2-01006] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OBFC1 antibody, and then analyzed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

OBFC1 Antibody (2E4) Summary

Immunogen
Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
OBFC1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OBFC1 Antibody (2E4)

  • AAF44
  • AAF-44
  • alpha accessory factor 44
  • bA541N10.2
  • CST complex subunit STN1
  • oligonucleotide/oligosaccharide-binding fold containing 1
  • Oligonucleotide/oligosaccharide-binding fold-containing protein 1
  • replication protein A 32 kDa subunit
  • RP11-541N10.2
  • RPA-32
  • STN1FLJ22559
  • Suppressor of cdc thirteen homolog

Background

OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OBFC1
Entrez