NYS48 [p Ser70] Antibody (NBP2-19128)

Images

 
Western Blot: NYS48 [p Ser70] Antibody [NBP2-19128] - Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells. Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT. Lane 3: ...read more
Western Blot: NYS48 [p Ser70] Antibody [NBP2-19128] - Lane 1: 293T Null. Lane 2: 293T WT Hice1. Lane 3: 293T S70A Hice1. Load: 14 ul per lane. Primary antibody: HICE1 pS70 antibody at 0.75 ug/mL overnight at 4C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NYS48 [p Ser70] Antibody Summary

Immunogen
Phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1. Protein accession number NP_219485.1. The target domain of reactivity is Internal.
Modification
p Ser70
Specificity
This antibody is directed against the phosphorylated form of human Hice1 protein at the pS70 residue. NBP2-19128 was cross adsorbed against the non-phosphorylated form of the immunizing peptide. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAUS8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1.22 ug/ml
  • ELISA 1:17000-1:45000
Application Notes
Tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Reactivity Notes

Reactive in Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NYS48 [p Ser70] Antibody

  • DGT4
  • HAUS augmin-like complex subunit 8
  • HAUS augmin-like complex, subunit 8
  • HEC1/NDC80 interacting, centrosome associated 1
  • Hec1-interacting and centrosome-associated 1
  • HICE1HEC1/NDC80-interacting centrosome-associated protein 1
  • MGC20533
  • NY-SAR-48
  • Sarcoma antigen NY-SAR-48

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HAUS8
Uniprot