NXNL2 Antibody (2B11)

Images

 
Western Blot: NXNL2 Antibody (2B11) [NBP2-02605] Analysis of extracts (35ug) from 9 different cell lines by using anti-NXNL2 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: NXNL2 Antibody (2B11) [NBP2-02605] - Immunofluorescent staining of HeLa cells using anti-NXNL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NXNL2 Antibody (2B11) [NBP2-02605] - Staining of paraffin-embedded Human pancreas tissue using anti-NXNL2 mouse monoclonal antibody.
Flow Cytometry: NXNL2 Antibody (2B11) [NBP2-02605] - Analysis of Jurkat cells, using anti-NXNL2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: NXNL2 Antibody (2B11) [NBP2-02605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NXNL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: NXNL2 Antibody (2B11) [NBP2-02605] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NXNL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NXNL2 Antibody (2B11) [NBP2-02605] - Staining of paraffin-embedded Human Kidney tissue using anti-NXNL2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: NXNL2 Antibody (2B11) [NBP2-02605] - Staining of paraffin-embedded Human liver tissue using anti-NXNL2 mouse monoclonal antibody.
Flow Cytometry: NXNL2 Antibody (2B11) [NBP2-02605] - Analysis of Hela cells, using anti-NXNL2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
2B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NXNL2 Antibody (2B11) Summary

Immunogen
Full length human recombinant protein of human NXNL2 (NP_660326) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NXNL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50

Reactivity Notes

Human, Monkey

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NXNL2 Antibody (2B11)

  • C9orf121
  • chromosome 9 open reading frame 121
  • nucleoredoxin-like 2
  • nucleoredoxin-like protein 2
  • RDCVF2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NXNL2 Antibody (NBP2-02605). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for antibodies that distinguish between NXNL1 and NXNL2. There is some overlap of sequence in the middle of the protein, so I would need antibodies generated against the C-terminus. Can you tell me if your antiNXNL2 antibody cross reacts with NXNL1?
    • NXNL1 and NXNL2 only have 32% homology among each other, so the chances that this antibody will cross-react with NXNL1 is fairly slim. However, this antibody was raised against the full-length NXNL2, and we do not epitope map, so there is always a slight possibility that this could recognize a portion of the sequence that is homologous with NXNL1. Given that there is only a few amino acids here and there that are homologous between the two, I believe that cross-reactivity chances are minimal. Unfortunately, we have not yet tested this ourselves for cross-reactivity to NXNL1 and cannot guarantee that there will be no cross reactivity.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NXNL2
Entrez