NXF3 Antibody

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Western Blot: NXF3 Antibody [NBP1-00962] - Western blot (WB) analysis of NXF3 (N104) antibody in extracts from MCF-7 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-00962
    • Availability
      Product Discontinued

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NXF3 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 70-121 of Human NXF3.
Specificity
NXF3 (N104) detects endogenous levels of NXF3 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
NXF3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of NXF3 (N104) pAb in extracts from MCF-7 cells.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This antibody is reactive against Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NXF3 Antibody

  • nuclear RNA export factor 3
  • TAPL3
  • TAPL-3
  • TAP-like protein 3

Background

Nuclear export factor (NXF) proteins belong to an evolutionarily conserved family of proteins which are characterized by a leucine-rich-repeat domain(LRR) followed by a region known as the Nuclear Transport Factor 2 (NTF2)-like domain. The NXF family includes TAP1 (NXF1) and NXF2-5. TAP1 mediates the export of constitutive transport element (CTE)-containing simian type D retroviral RNAs through direct binding to the CTE. NXF2 binds RNA and localizes to the nuclear envelope, where it exhibits RNA export activity. NXF3 does not bind RNA nor localize to the nuclear rim, and NXF3 does not exhibit RNA export activity. NXF5 binds RNA and localizes in the form of granules in the cell body and neurites of mature hippocampal neurons. TAP1, NXF2 and NXF5 form heterodimers with RNA nuclear export-associated protein p15 (NXT). The human NXF gene cluster maps to Xcen-NXF5-NXF2-NXF4-NXF3-Xqter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Diseases for NXF3 Antibody (NBP1-00962)

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Bioinformatics

Gene Symbol NXF3
Entrez