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NUDT9 Overexpression Lysate (Denatured)

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Summary
Reactivity HuSpecies Glossary
Applications WB

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NUDT9 Overexpression Lysate (Denatured) Summary

Description
Plasmid: pCMV-NUDT9 full-length
Gene
NUDT9

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Lysate Details for Array

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-NUDT9 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NUDT9 Overexpression Lysate (Denatured)

  • ADP-ribose diphosphatase
  • ADP-ribose phosphohydrolase
  • ADP-ribose pyrosphosphatase NUDT9
  • ADPR-PPase
  • EC 3.6.1.13
  • MGC3037
  • mitochondrial
  • nucleoside diphosphate linked moiety X-type motif 9
  • nudix (nucleoside diphosphate linked moiety X)-type motif 9
  • Nudix motif 9
  • NUDT10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TRPM2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Staining of rat hippocampus using NB110-81601. TRPM2 is stained red, GFAP is stained green and nuclei staining is blue. Photo courtesy of Dr. Ji-Zhong Bai, The University of Auckland, New Zealand.
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Clusterin/APOJ
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Clusterin was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Clusterin was detected in perfusion fixed frozen sections of mouse intestine using Goat Anti-Mouse Clusterin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2747) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Immunohistochemistry: AF4 Antibody [NBP1-28728] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- AF4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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NUDT11 Antibody
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Western Blot: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: ADP-Sugar Pyrophosphatase/NUDT5 Antibody [NBP1-83131] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
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CRAT Antibody
NBP1-86616
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GLYAT Antibody
NBP2-55298
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Bioinformatics

Gene Symbol NUDT9