Nrf2 Antibody (EP1808Y)

Product Discontinued

Nrf2 Antibody (EP1808Y) Summary

• Immunogen: A synthetic peptide corresponding to residues near the C-terminus of human Nrf2 was used as an immunogen. The sequence maps between amino acids 580 and 605.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFE2L2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:20
  • Western Blot 1:1000-10000
• Application Notes: In Western blot this antibody detects a band at approximately 100 kDa.Use in IHC-F was reported in the scientific literature (PMID: 19734940). Use in Chip and CHIPseq was reported in the scientific literature (PMID: 22581777).
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25769424)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Nrf2 Antibody (EP1808Y)

• HEBP1
• NFE2L2
• NF-E2-related factor 2
• NFE2-related factor 2
• NRF2 erythroid derived 2, like 2
• Nrf2
• nuclear factor (erythroid-derived 2)-like 2
• nuclear factor erythroid 2-related factor 2
• nuclear factor erythroid-derived 2-like 2

Background

Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress (1). Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin (2). Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nrf2 Antibody (NB100-80011)(7)

Publications using NB100-80011

• Li L, Shen C, Nakamura E et al. SQSTM1 is a pathogenic target of 5q copy number gains in kidney cancer. Cancer Cell. 2013-12-09 [PMID: 24332042] (WB, IP, Human)
• Wojewoda M, Duszy?ski J, Szczepanowska J et al. Antioxidant defence systems and generation of reactive oxygen species in osteosarcoma cells with defective mitochondria: effect of selenium. Biochim Biophys Acta 2010-06-01 [PMID: 20138159] (WB)
• Yang L, Calingasan NY, Thomas B et al. Neuroprotective effects of the triterpenoid, CDDO methyl amide, a potent inducer of Nrf2-mediated transcription. PLoS One 2009-06-01 [PMID: 19484125] (WB)
• Carvalho A, Franklin G, Dias A et al. Methanolic extract of Hypericum perforatum cells elicited with Agrobacterium tumefaciens provides protection against oxidative stress induced in human HepG2 cells. Industrial Crops and Products. 2014-01-01
• Zhang Z, Li G, Szeto SS et al. Examining the neuroprotective effects of protocatechuic acid and chrysin on in vitro and in vivo models of Parkinson's disease. Free Radic. Biol. Med. 2015-03-10 [PMID: 25769424] (WB, ICC/IF, Rat)
• Carvalho AC, Gomes AC, Pereira-Wilson C, Lima CF. Redox-dependent induction of antioxidant defenses by phenolic diterpenes confers stress tolerance in normal human skin fibroblasts: Insights on replicative senescence Free Radic. Biol. Med. 2015-03-02 [PMID: 25744415] (WB, Human)
• Milhollen MA, Traore T, Adams-Duffy J et al. MLN4924, a NEDD8-activating enzyme inhibitor, is active in diffuse large B-cell lymphoma models: rationale for treatment of NF-?B-dependent lymphoma ,Blood 116 (2010) 1515-1523. Biochim Biophys Acta 1797 890-896. 2010-01-01 [PMID: 20525923]

FAQs for Nrf2 Antibody (NB100-80011). (Showing 1 - 1 of 1 FAQs).

1. I would like to know the specific epitope of NRF2 this antibody, NB100-80011 binds to?
   • The sequence we used is proprietary, but it maps between amino acids 580 and 605 of human Nrf2, at the C-terminus

Bioinformatics

• Gene Symbol: NFE2L2
• Entrez:
  • 4780(Human)
• Uniprot:
  • Q16236()