NQO-1 Antibody


Western Blot: NQO-1 Antibody [NBP1-51228] - Analysis of NQO1 (F252) pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate
Western Blot: NQO-1 Antibody [NBP1-51228] - Extracts from HeLa cells

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
1.0 mg/ml

Order Details

NQO-1 Antibody Summary

Synthetic peptide, corresponding to amino acids 220-370 of Human NQO1.
NQO1 (F252) pAb detects endogenous levels of NQO1 protein.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Phosphate buffered saline (PBS) approx. pH 7.2.
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
Western blot (WB) analysis of NQO1 (F252) pAb in extracts from HeLa cells The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa

Alternate Names for NQO-1 Antibody

  • Azoreductase
  • DHQU
  • DIA4
  • DIA4azoreductase
  • diaphorase (NADH/NADPH) (cytochrome b-5 reductase)
  • Diaphorase-4
  • Dioxin-inducible 1
  • DTD
  • DT-diaphorase
  • Menadione Reductase
  • NAD(P)H dehydrogenase [quinone] 1
  • NAD(P)H dehydrogenase, quinone 1
  • NAD(P)H:menadione oxidoreductase 1
  • NAD(P)H:Quinone acceptor oxidoreductase type 1
  • NAD(P)H:quinone oxidoreductase 1
  • NAD(P)H:quinone oxireductase
  • NMOR1
  • NMOR1diaphorase-4
  • NQO1
  • Phylloquinone reductase
  • QR1
  • Quinone Reductase 1


NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein,is ubiquitously expressed, but the expression level varies among tissues.NQO1 gene expression is coordinately induced in response to xenobiotics,antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-utylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol NQO1

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