Notch-4 Antibody (HMN4-14) [PE] Summary
The immunogen for this antibody is NOTCH4.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C in the dark.
PE (A=488, E=575)
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
Protein A or G purified
This HMN4-14 antibody has been tested by flow cytometric analysis of mouse splenocytes and Notch-4-transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Mouse. May react with Rat.
Alternate Names for Notch-4 Antibody (HMN4-14) [PE]
- neurogenic locus notch homolog protein 4
- notch 4
- Notch homolog 4
This HMN4-14 monoclonal antibody reacts with mouse Notch4, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by ?-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, forms a ternary complex with the DNA-binding transcription factor CSL (CBF-1, Su(H), Lag-1) and the coactivator Mastermind, and ultimately activates transcription.
Notch 4 has been shown to be expressed in CD8+ splenic dendritic cells, endothelial cells (e.g. vascular smooth muscle cells), and macrophages. A truncated, constitutively active form of Notch 4 designated Int3 also exists in mice as a result of the mouse mammary tumor virus (MMTV) insertion into the Notch 4 gene. Composed of the transmembrane and intracellular domains of Notch 4, Int3 has been reported to be involved in mammary gland development and tumorigenesis in the mouse. Finally, studies have shown Notch 4 involvement in myeloid proliferation, hematopoiesis, and embryonic endothelial development.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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