Notch-4 Antibody (HMN4-14) [PE]



Product Details

Product Discontinued
View other related Notch-4 Primary Antibodies

Order Details

    • Catalog Number
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Notch-4 Antibody (HMN4-14) [PE] Summary

The immunogen for this antibody is NOTCH4.
Armenian Hamster
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:10-1:1000
Application Notes
This HMN4-14 antibody has been tested by flow cytometric analysis of mouse splenocytes and Notch-4-transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Mouse. May react with Rat.

Packaging, Storage & Formulations

Store at 4C in the dark.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.2 mg/ml
Protein A or G purified

Alternate Names for Notch-4 Antibody (HMN4-14) [PE]

  • INT3
  • neurogenic locus notch homolog protein 4
  • notch 4
  • Notch homolog 4
  • NOTCH3
  • Notch4
  • Notch-4


This HMN4-14 monoclonal antibody reacts with mouse Notch4, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling. Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by ?-secretase. The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, forms a ternary complex with the DNA-binding transcription factor CSL (CBF-1, Su(H), Lag-1) and the coactivator Mastermind, and ultimately activates transcription.

Notch 4 has been shown to be expressed in CD8+ splenic dendritic cells, endothelial cells (e.g. vascular smooth muscle cells), and macrophages. A truncated, constitutively active form of Notch 4 designated Int3 also exists in mice as a result of the mouse mammary tumor virus (MMTV) insertion into the Notch 4 gene. Composed of the transmembrane and intracellular domains of Notch 4, Int3 has been reported to be involved in mammary gland development and tumorigenesis in the mouse. Finally, studies have shown Notch 4 involvement in myeloid proliferation, hematopoiesis, and embryonic endothelial development.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, KO
Species: Rt
Applications: WB, Flow, IHC, Block, CyTOF-ready
Species: Hu, Mk
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Mu
Applications: WB, ICC
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu, Pm
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, ICC
Species: Mu
Applications: WB
Species: Hu
Applications: ICC/IF
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
Species: Mu
Applications: Flow

Publications for Notch-4 Antibody (NBP2-00270) (0)

There are no publications for Notch-4 Antibody (NBP2-00270).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Notch-4 Antibody (NBP2-00270) (0)

There are no reviews for Notch-4 Antibody (NBP2-00270). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Notch-4 Antibody (NBP2-00270) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Notch-4 Products

Bioinformatics Tool for Notch-4 Antibody (NBP2-00270)

Discover related pathways, diseases and genes to Notch-4 Antibody (NBP2-00270). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Notch-4 Antibody (NBP2-00270)

Discover more about diseases related to Notch-4 Antibody (NBP2-00270).

Pathways for Notch-4 Antibody (NBP2-00270)

View related products by pathway.

PTMs for Notch-4 Antibody (NBP2-00270)

Learn more about PTMs related to Notch-4 Antibody (NBP2-00270).

Research Areas for Notch-4 Antibody (NBP2-00270)

Find related products by research area.

Blogs on Notch-4

There are no specific blogs for Notch-4, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Notch-4 Antibody (HMN4-14) [PE] and receive a gift card or discount.


Gene Symbol NOTCH4