Notch-1 Antibody

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Western Blot: Notch-1 Antibody [NBP1-51487] - Analysis of extracts from NIH/3T3 cells.
Immunohistochemistry-Paraffin: Notch-1 Antibody [NBP1-51487] - Human brain tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51487
    • Availability
      Product Discontinued

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Notch-1 Antibody Summary

Immunogen
The immunogen for this antibody was a synthetic Cleaved-Notch 1 (V1754) peptide.
Specificity
Cleaved-Notch 1 (V1754) pAb detects endogenous levels of Cleaved-Notch 1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
NOTCH1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Cleaved-Notch 1 (V1754) pAb in extracts from NIH/3T3 cellsImmunohistochemistry (IHC) analyzes of Cleaved-Notch 1 (V1754) pAb in paraffin-embedded human brain tissue.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Notch-1 Antibody

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • Notch1
  • Notch-1
  • TAN1
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Background

Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1753 and Val1754. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Notch-1 Antibody (NBP1-51487)

Discover related pathways, diseases and genes to Notch-1 Antibody (NBP1-51487). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Notch-1 Antibody (NBP1-51487)

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Blogs on Notch-1.

Notch1 - A multifunctional transmembrane receptor
Notch1 is a member of the Notch family of Type 1 single-pass transmembrane proteins that share an extracellular domain of multiple epidermal growth factor-like (EGF) repeats. Notch family members play key roles in a variety of developmental process...  Read full blog post.

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Bioinformatics

Gene Symbol NOTCH1
Entrez
Uniprot