Normetanephrine ELISA Kit (Colorimetric)

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ELISA: Normetanephrine ELISA Kit (Colorimetric) [KA1893] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Applications ELISA
Suitable Sample Type
Urine
Standard Curve Range
40 to 5200 ng/mL
Sensitivity
23 ng/mL

Order Details

Normetanephrine ELISA Kit (Colorimetric) Summary

Standard Curve Range
40 to 5200 ng/mL
Sensitivity
23 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 20.35%
Intra-Assay
CV% < 17.5%
Spike Recovery
1.03
Sample Volume
25 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Normetanephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of free Normetanephrine in urine.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins conjugated withperoxidase.
  2. Control 1: Ready to use, refer to QC-Report for expected value and acceptable range.
  3. Control 2: Ready to use, refer to QC-Report for expected value and acceptable range.
  4. Normetanephrine Antiserum: Ready to use, rabbit anti-Normetanephrine antibody, yellowcoloured.
  5. Normetanephrine Microtiter Strips: Ready to use, antigen precoated microwell plate in aresealable yellow pouch with desiccant.
  6. Hydrochloric Acid: Ready to use, 0.25 M hydrochloric acid, yellow coloured.
  7. Acylation Diluent: Ready to use, dimethysulfoxide.
  8. Acylation Buffer: Ready to use, TRIS buffer.
  9. Reaction Tubes: Ready to use, in a resealable pouch.
  10. Standard A: Ready to use, 0 ng/mL (0 nmol/L)
  11. Standard D: Ready to use, 300 ng/mL (1638 nmol/L)
  12. Standard C: Ready to use, 90 ng/mL (491 nmol/L)
  13. Standard B: Ready to use, 30 ng/mL (164 nmol/L)
  14. Standard E: Ready to use, 900 ng/mL (4914 nmol/L)
  15. Standard F: Ready to use, 3000 ng/mL (16380 nmol/L)
  16. Wash Buffer Concentrate (50X): Buffer with a non-ionic detergent and physiological pH.
  17. Stop Solution: Ready to use, 0.25 M sulfuric acid.
  18. Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine, substratebuffer and hydrogen peroxide.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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