Recombinant Human Noggin Protein Summary
A biologically active protein to NOG.
Source: E. coli
Amino Acid Sequence: MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPEDRPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQRLSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSVPEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSCPurity Specification:46.2kDa, >95%SDS-PAGE Results:25.5kDa, 81.8% ED50 Spec:3 ng/mLED50 Actual:0.872 ug/mLCells:ATDC-5
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
Noggin Protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4- induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 x 105 IU/mg in the presence of 5 ng/ml rHuBMP-4.
| Protein/Peptide Type
Biologically Active Protein
>95% pure by SDS-PAGE
Less than 1 EU/ug of endotoxin as determined by LAL method.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
>95% pure by SDS-PAGE
Reconstitute with 10 mM HAc to a final concentration of 0.1 - 1.0 mg/ml.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human Noggin Protein
- symphalangism 1 (proximal)
- synostoses (multiple) syndrome 1
Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF- beta family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature human Noggin shares 99 %, 99 %, 98 %, 97 % and 89 % a.a. sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Species: Hu, Mu
Applications: WB, IHC
Applications: WB, IHC
Applications: IHC, IHC-P
Species: Hu, Rt
Applications: IHC, CyTOF-ready, ICC, ICFlow
Applications: Func, PAGE
Publications for Noggin Protein (NBP2-35079) (0)
There are no publications for Noggin Protein (NBP2-35079).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Noggin Protein (NBP2-35079) (0)
There are no reviews for Noggin Protein (NBP2-35079).
By submitting a review earn points towards our Rewards Program.
- 250 points for product review
- 500 additional points for an image with your product review
- Double points (500) if you are the first to review this product
- Double points (1000) if you are the first to review this product with an image
FAQs for Noggin Protein (NBP2-35079) (0)
Additional Noggin Products
Related Products by Gene
Bioinformatics Tool for Noggin Protein (NBP2-35079)
Discover related pathways, diseases and genes to Noggin Protein (NBP2-35079). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Noggin.
Five Funny Protein Names: Infographic
Some proteins have the strangest names. Check out our list for some of the funniest ones and learn more about each protein including their function, gene symbol, molecular weight and family.Novus Biologicals offers reagents for each target:Merli... Read full blog post.