nNOS Antibody

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Western Blot: nNOS Antibody [NB100-92267] - Western blot (WB) analysis of nNOS (Y846) antibody in extracts from A549 cells.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-92267
    • Availability
      Product Discontinued

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nNOS Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 815-862 of Human NOS1.
Clonality
Polyclonal
Host
Rabbit
Gene
NOS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of nNOS (Y846) pAb in extracts from A549 cells.
Theoretical MW
43125149160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
nNOS Lysate (NBL1-13719)

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for nNOS Antibody

  • bNOS
  • brain
  • Constitutive NOS
  • EC 1.14.13.39
  • IHPS1
  • Neuronal NOS
  • nitric oxide synthase 1 (neuronal)
  • nNOS
  • N-NOS
  • nNOSNOS type I
  • NOS
  • NOS1
  • Peptidyl-cysteine S-nitrosylase NOS1

Background

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. nNOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct nNOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including ncNOS (also designated NOS1) whose activity was first identified in neurons and maps at 12q24.2, and ecNOS (also designated NOS3), first identified in endothelial cells and mapping at 7q35-36. The inducible form of nNOS, iNOS (also designated NOS2), is Ca2+-independent, expressed in a broad range of cell types and maps to 17cen -q12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for nNOS Antibody (NB100-92267)

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Diseases for nNOS Antibody (NB100-92267)

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Bioinformatics

Gene Symbol NOS1
Entrez